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In more length: the term \"HTML5\" is widely used as a buzzword to refer to modern web technologies, many of which (though by no means all) are developed at the WHATWG. This document is one such; others are available from the WHATWG Standards overview.
Some conformance requirements are phrased as requirements on elements, attributes, methods or objects. Such requirements fall into two categories: those describing content model restrictions, and those describing implementation behavior. Those in the former category are requirements on documents and authoring tools. Those in the second category are requirements on user agents. Similarly, some conformance requirements are phrased as requirements on authors; such requirements are to be interpreted as conformance requirements on the documents that authors produce. (In other words, this specification does not distinguish between conformance criteria on authors and conformance criteria on documents.)
Some attributes, called enumerated attributes, take on a finite set of states. The state for such an attribute is derived by combining the attribute's value, a set of keyword/state mappings given in the specification of each attribute, and two possible special states that can also be given in the specification of the attribute. These special states are the invalid value default and the missing value default.
If the attribute has a missing value default state defined, then return that missing value default state.Otherwise, return no state. If the attribute's value is an ASCII case-insensitive match for one of the keywords defined for the attribute, then return the state represented by that keyword.If the attribute has an invalid value default state defined, then return that invalid value default state.Return no state. For authoring conformance purposes, if an enumerated attribute is specified, the attribute's value must be an ASCII case-insensitive match for one of the conforming keywords for that attribute, with no leading or trailing whitespace.
If there is only one keyword mapping to the given state, then it is that keyword.If there is only one conforming keyword mapping to the given state, then it is that conforming keyword.Otherwise, the canonical keyword for the state will be explicitly given in the specification for the attribute. 2.3.4 Numbers 2.3.4.1 Signed integers A string is a valid integer if it consists of one or more ASCII digits, optionally prefixed with a U+002D HYPHEN-MINUS character (-).
Let input be the string being parsed.If input is the empty string, then return an error.Strip leading and trailing ASCII whitespace from input.If input is an ASCII case-insensitive match for the string \"transparent\", then return an error. If input is an ASCII case-insensitive match for one of the named colors, then return the simple color corresponding to that keyword. [CSSCOLOR]
A set of space-separated tokens is a string containing zero or more words (known as tokens) separated by one or more ASCII whitespace, where words consist of any string of one or more characters, none of which are ASCII whitespace.
If there is no base element that has an href attribute in the Document, then return the Document's fallback base URL.Otherwise, return the frozen base URL of the first base element in the Document that has an href attribute, in tree order. A URL matches about:blank if its scheme is \"about\", its path contains a single string \"blank\", its username and password are the empty string, and its host is null.
Let position be a pointer into s, initially pointing at the start of the string.Loop: Find the first seven characters in s after position that are an ASCII case-insensitive match for the word \"charset\". If no such match is found, return nothing.Skip any ASCII whitespace that immediately follow the word \"charset\" (there might not be any).If the next character is not a U+003D EQUALS SIGN (=), then move position to point just before that next character, and jump back to the step labeled loop.Skip any ASCII whitespace that immediately follow the equals sign (there might not be any). Process the next character as follows:
The attribute's invalid value default is the Anonymous state, and its missing value default is the No CORS state. For the purposes of reflection, the canonical keyword for the Anonymous state is the anonymous keyword.
IDL attributes of type DOMString or DOMString that reflect enumerated content attributes can be limited to only known values. Per the processing models below, those will cause the getters for such IDL attributes to only return keywords for those enumerated attributes, or the empty string or null.
If the content attribute is not in any state (e.g. the attribute is missing and there is no missing value default), or the content attribute is in a state with no associated keyword value, then return the empty string.Return the canonical keyword for the state of the content attribute. Otherwise:
Assert: the content attribute is an enumerated attribute.Assert: the IDL attribute is limited to only known values.Assert: the content attribute is in some state.If the content attribute is in a state with no associated keyword value, then return null.Return the canonical keyword for the state of the content attribute. The setter steps are:
The serialization steps may need to perform a sub-serialization. This is an operation which takes as input a value subValue, and returns StructuredSerializeInternal(subValue, forStorage, memory). (In other words, a sub-serialization is a specialization of StructuredSerializeInternal to be consistent within this invocation.)
The deserialization steps may need to perform a sub-deserialization. This is an operation which takes as input a previously-serialized Record subSerialized, and returns StructuredDeserialize(subSerialized, targetRealm, memory). (In other words, a sub-deserialization is a specialization of StructuredDeserialize to be consistent within this invocation.)
If name is applet, bgsound, blink, isindex, keygen, multicol, nextid, or spacer, then return HTMLUnknownElement.If name is acronym, basefont, big, center, nobr, noembed, noframes, plaintext, rb, rtc, strike, or tt, then return HTMLElement.If name is listing or xmp, then return HTMLPreElement.Otherwise, if this specification defines an interface appropriate for the element type corresponding to the local name name, then return that interface.If other applicable specifications define an appropriate interface for name, then return the interface they define.If name is a valid custom element name, then return HTMLElement.
Total protein was extracted by lysing cells in RIPA buffer containing protease inhibitor. Protein samples were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride (PVDF) membranes. After blocking with 5% non-fat milk or 3% BSA in TBS-T, membranes were incubated with the primary antibody. The following antibodies were used: GPR55 (1:1000, Cayman, USA), ERK1 (1:2000, Santa Cruz, USA), p-ERK1/2 (1:2000, SantaCruz,USA), anti-cyclinD1 (1:2000), MMP2 (1:1000, Proteintech.,USA), MMP9 (1:1000, Proteintech Group,USA), RB(1:1000, Proteintech Group,USA), Tubulin (1:3000, Abcam, USA) and β-actin (1:3000, Bioword, Britain) TBK1(1:2000, Upstate Biotechnologies/Millipore, Billerica, MA) and goat-anti-rabbit IgG conjugated to horseradish peroxidase (HRP) (1:5000, Santa Cruz, USA), which was used as the secondary antibody. Cells were seeded on 10-cm cell culture plates, grown to 80% confluency, and serum starved overnight. To evaluate the activity of RhoA, the cells washed twice with ice-cold TBS and harvested on ice with 500 μl of 1X MLB lysis buffer (Upstate Biotechnology Inc.). Lysates were then clarified by centrifugation at 14,000 g for 10 min. The cell lysates were incubated with 30 μg of GST-RBD-agarose beads to precipitate GTP-bound RhoA. The beads were pelleted by brief centrifugation (12,000 g for 30 s), and washed 3 times with lysis buffer. Samples were denatured in sample buffer, boiled for 5 min at 90C, and resolved using a 12% SDS-PAGE gel. Bound RhoA was detected by Western blot using a monoclonal anti-RhoA antibody, followed by secondary antibody incubation. Bands were visualized using the enhanced chemiluminescence kit (Santa Cruz Biotechnology, Santa Cruz, CA). Target signals were quantified by BandScan software (Bio-Rad, Hercules, CA) and defined as the ratio of target protein relative to β-actin or β-tubulin. 153554b96e
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